As a study on the acid protease production by Rhizopus japoricus S-62, the culture conditions for the enzyme production and characteristics of the crude enzyme were investigated. The results are summarized as follows:
1. The optimum conditions for solid culture on wheat bran were 48 hrs of culture period and 100∼120% addition of tap water.
2. Of the several media, wheat bran medium was the most excellent in the enzyme production.
3. The addition of sucrose, fructose, NH₄Cl and NaH₂PO₄ on wheat bran, respectively, increased largely the enzyme production.
4. The optimum pH for the enzyme action was pH 2.4∼2.6, the optimum temperature was about 40℃, and the stable pH range was pH 2.5∼5.0, the enzyme was stable below 40℃ and was inactivated abruptly above 40℃.
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